Office: HAC 416
Lab: HAC 417
A.B. in Chemistry and Mathematics, Vassar College. PIs: Prof. Miriam Rossi and Prof. Joseph Tanski
PhD. in Biological Chemistry, Massachusetts Institute of Technology. PIs: Prof. Catherine L. Drennan and Prof. Collin M. Stultz.
Postdoctoral Research Assistant, University of California, Berkeley and The Scripps Research Institute. PI: Prof. Michael A. Marletta.
Work in my lab uses a variety of biochemical techniques to understand protein function and X-ray crystallography to analyze protein structure. In general, I am interested in how cells sense and respond to their environment.
Exploring Protein Structural Impacts of Unnatural Amino Acids
The incorporation of unnatural amino acids (UAAs) into proteins to probe protein structure, function, and kinetics has become rather commonplace. While it is generally accepted that these amino acid substitutions do not cause significant disruptions to protein structure, relatively few structures with unnatural amino acids have been published. In collaboration with Prof. Scott Brewer's lab at F&M, we are using the green fluorescent protein (GFP) as a model system to obtain X-ray crystal structures of GFP with the vibrational reporter UAAs 4-cyano-L-phenylalanine or 4-azidomethyl-L-phenylalanine incorporated at a variety of sites in the protein. With these structures, we hope to provide a set of examples illustrating that the thoughtful incorporation of unnatural amino acids does not significantly effect protein structure, supporting their use as spectroscopic probes for studying protein structure and dynamics.
Dehalococcoides mccartyi Sensing of Halogenated Pollutants
One project in my lab is examining the mechanism by which a bacterium involved in the breakdown of the carcinogenic groundwater pollutants perchloroethene (PCE) and trichloroethene (TCE) sense these small molecules in the environment and turn on the appropriate dehalogenation machinery. We are working to identify the proteins involved in sensing PCE or TCE in the environment and relaying this signal to the cell. We will then characterize the signaling and DNA binding properties of these proteins and use X-ray crystallography to help illuminate the molecular details of how D. mccartyi can differentiate between the two very similar compounds – PCE and TCE – which vary only in a single chlorine atom.
Grants & Awards
NIH R15 Academic Research Enhancement Award Grant entitled: "Monitoring and Tuning a Gas-Binding Heme Protein with Unnatural Amino Acids." 2016 - 2019
Research Corporation Cottrell College Science Award for our project entitled: "Sensing of Halogenated Pollutants by Dehalococcoides mccartyi." 2014 - 2016
NIH Ruth L. Kirschstein NRSA Postdoctoral Fellowship (F32) (declined)
Chair of 2011 Bioinorganic Chemistry Gordon Graduate Research Seminar, Ventura, CA
Sigma Xi, Vassar College Chapter
Chemistry Department Award to Graduating Senior, Vassar College
ACS College Recognition Award, Vassar College
F&M Undergraduate co-authors are in bold and corresponding authors are indicated with an asterisk.
Kearney, C.; Olenginski, L. T., Hirn, T. D.; Fowler, G. D.; Tariq, D.; Brewer, S. H.*; Phillips-Piro, C. M.* Exploring Local Solvation Environments of a Heme Protein using the Spectroscopic Reporter 4-Cyano-L-phenylalanine. RSC Advances, 2018, 8, 13503-13512.
Hespen, C.W.; Bruegger, J.J.; Phillips-Piro, C. M.*; Marletta, M. A. Structural and Functional Evidence Indicates Selective Oxygen Signaling in Caldanaerobacter subterraneus H NOX. ACS Chemical Biology, 2016, DOI: 10.1021/acschembio.6boo431.
Dippel, A.B.; Olenginski, G.M.; Maurici, N.; Liskov, M. T.; Brewer, S. H.*; and Phillips-Piro, C.M.* Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structual study. Acta Cryst. D. 2016, D72, 121-130.
Tookmanian, E.M.; Phillips-Piro, C.M.*; Fenlon, E.E.*; and Brewer, S.H.* Azidoethoxyphenylalanine as a Vibrational Reporter and Click Chemistry Partner in Proteins. Chemistry–A European Journal. 2015, 21, 19096-19103.
Weinert, E.E.; Phillips-Piro, C. M.; Marletta, M. A. Porphyrin pi-Stacking in a Heme Protein Scaffold Tunes Gas Ligand Affinity. J. Inorg. Biochem. 2013, 127, 7-12.
Winter, M. B.; Klemm, P. J.; Phillips-Piro, C. M.; Raymond, K. N.; Marletta, M. A. Porphyrin Substituted H-NOX Proteins as High Relaxivity MRI Contrast Agents. Inorg. Chem. 2013 52 (5), 2277-2279.
Weinert, E. E.; Phillips-Piro, C. M.; Tran, R.; Mathies, R. A.; Marletta, M. A. Controlling Conformational Flexibility of an O2-Binding H-NOX Domain. Biochemistry 2011, 50, 6832-6840.
Phillips, C. M.; Stultz, C. M.; Drennan, C. L. Search for the nik Operon: How a Ligand-Responsive Transcription Factor Hunts for its DNA Binding Site. Biochemistry 2010, 49, 7757-7763.
Phillips, C. M.; Schreiter, E. R.; Stultz, C. M.; Drennan, C. L. Structural Basis of Low Affinity Nickel-Responsive Transcription Factor NikR from Escherichia coli. Biochemistry 2010, 49, 7830-7838.
Phillips, C. M.; Nerenberg, P. S.; Drennan, C. L.; Stultz, C. M. The Physical Basis for Metal Selectivity in Escherichia coli NikR. Journal of the American Chemical Society 2009, 131, 10220-10228.
Phillips, C. M.; Drennan, C. L. Nickel Regulatory Transcription Factor, NikR. Handbook of Metalloproteins; Messerschmidt, A., Ed.; John Wiley & Sons, Ltd. 2008.
Bohon, J.; Jennings, L. D.; Phillips, C. M.; Licht, S.; Chance, M. R. The Conformation of Functional Domains in the ClpA Hexamer : A Synchrotron Footprinting Study. Structure 2008, 16, 1157-1165.
Phillips, C. M.; Schreiter, E. R.; Guo, Y.; Wang, S. C.; Zamble, D. B.; Drennan, C. L. Structural Basis of the Metal Specificity for Nickel Regulatory Protein NikR. Biochemistry 2008, 47 (7), 1938-1946.
Caruso, F.; Pettinari, C.; Marchetti, F.; Natanti, P.; Phillips, C.; Tanski, J.; Rossi, M. Synthesis, Molecular Structure (X-ray and DFT), and Solution Behavior of Titanium 4-Acyl-5-pyrazolonates. Correlations with Related Antitumor b-Diketonato Derivatives. Inorganic Chemistry 2007, 46, 7553-7560.
Pettinari, C.; Caruso, F.; Zaffaroni, N.; Villa, R.; Marchetti, F.; Pettinari, R.; Phillips, C.; Tanski, J.; Rossi, M. Synthesis, Spectroscopy (IR, multinuclear NMR, ESI-MS), Diffraction, Density Functional Study and in vitro Antiproliferative Activity of Pyrazole-beta-diketone Dihalotin(IV) Compounds on 5 Melanoma Cell Lines. Journal of Inorganic Biochemistry 2006, 100, 58-69.
Pettinari, C.; Marchetti, F.; Pettinari, R.; Cingolani, A.; Rivarola, E.; Phillips, C.; Tanski, J.; Rossi, M.; Caruso, F. Tin(II) and Lead(II) 4-Acyl-5-pyrazolonates: Synthesis, Spectroscopy (IR, 1H, 13C, and 119Sn NMR, 119Sn Mossbauer and ESI-MS) and X-ray Structural Characterization. European Journal of Inorganic Chemistry 2004, 17, 3484-3497.
2020 - Darcy Harris, Nicolette Runko
2018 - Trexler Hirn, Maggie Luo
2017 - Caroline Kearney, Juliana Piacentini, Nicole Savidge
2016 - Nicole Maurici, Daniyal Tariq, Jordan Alter, Carolina Giraldo
2015 - Gregory Olenginski, Lukasz Olenginski, Elise Tookmanian
2014 - Andrew Dippel, Austin Zimmet
2013 - Brittany Sembler, Melanie Liskov
CHM112 : General Chemistry II Lecture & Lab
CHM351 : Introductory Biochemistry Lecture & Lab
CHM112 : General Chemistry II Lecture & Lab
CHM371 : Introductory Biochemistry Lecture & Lab
CHM111: General Chemistry I Lecture & Lab
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